Fig 1: The miRNA-132/212 deletion alters protein levels of a7-nAChR and pERK in mouse hippocampus. Total cell lysates from male mouse bilateral hippocampi were analysed by Western blot to determine the protein expression levels of a7-nAChR, ERK and pERK. A, Representative immunoblots and relative levels of a7-nAChR protein together with corresponding GAPDH levels, indicating significant increase in protein levels of a7-nAChR in the hippocampus of miRNA-132/212-/- mice, compared with wild-type mice. B, Representative immunoblots and relative levels of ERK1 (p44) and ERK2 (p41) protein together with corresponding GAPDH levels in the hippocampus of miRNA-132/212-/- mice. Analysis showed no changes in protein levels of ERK compared with wild-type mice. C, Representative immunoblots and relative levels of pERK and GAPDH levels indicating significant decrease in protein levels of pERK in the hippocampus of miRNA-132/212-/- mice compared with wild-type mice. No significant differences were observed in protein levels of pERK. D, Representative immunoblots and relative levels of MeCP2 protein together with corresponding GAPDH levels, indicating no significant differences in protein levels of MeCP2 in the hippocampus of miRNA-132/212-/- mice, compared with wild-type mice. Results are shown relative to GAPDH. Data are expressed as mean ± SD. n = 5 per group. WT, wild type; kDa, kilodalton. Please see details for the statistics on the main text